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A technician is diagnosing an issue with a new T1 connection. The router is configured, the cable is connected, but the T1 is down.
A security analyst has a sample of malicious software and needs to know what the sample does. The analyst runs the sample in a carefully controlled and monitored virtual machine to observe the software behavior.
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A security analyst is reviewing packet captures from a system that was compromised. The system was already isolated from the network, but it did have network access for a few hours after being compromised.
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Software, and procedures in case of a catastrophic failure of the primary datacenter. The Chief Information Officer (CIO) is concerned because this contract does not include a long-term strategy for extended outages.
The computer incident response team at a multinational company has determined that a breach of sensitive data has occurred in which a threat actor has compromised the organization’s email system. Per the incident response procedures, this breach requires notifying the board immediately.
However, I realised that most of the time that interrupts the flow of the reading of the thread if you pick it up later. This is why I decided to mostly move the threads to a later day so the flow is not interrupted when you reach that thread.
A technician is investigating a server performance issue. The technician has gathered the server utilization statistics.
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A network technician is working on a proposal for email migration from an on-premises email system to a vendor-hosted email in the cloud. The technician needs to explain to management what type of cloud model will be utilized with the cloud-hosted email.
A pharmaceutical company's marketing team wants to send out notifications about new products to alert users of recalls and newly discovered adverse drug reactions. The team plans to use the names and mailing addresses that users have provided.
Importantly, we also demonstrated that WNT6 is a novel biomarker of GBM prognosis, as patients with WNT6-high tumors from a variety of independent datasets presented a significantly shorter OS than WNT6-low tumors (Figure 7, Table 1, and Figure S7), a clinical finding that is in agreement with our in vivo data (Figure 5 and Figure S3I-J). This might be linked to the WNT6 oncogenic activities, such as increased invasion/migration hampering complete tumor resection, increased tumor relapse probability due to its roles in GSC maintenance (Figure 4 and Figure S3F-H), and the response to TMZ treatment (Figure 3), which is still the gold-standard chemotherapeutic drug used for GBM patients. These findings fit well with previous reports describing that WNT6 increases the chemo-resistance of gastric and bladder cancer, and associates with the overall and disease-free survivals of esophageal squamous cell carcinoma patients . Together, these data implicate WNT6 as a novel prognostic biomarker and putative therapeutic target for GBM patients. The fact that WNT6 levels can be easily assessed at the mRNA or protein levels with routine molecular biology techniques may also facilitate the widespread implementation of this biomarker in the clinical settings.
WNT6 influences the activation status of WNT, SFK, and STAT pathways in GBM. Phospho-kinase arrays were performed to evaluate the phosphorylation status of cancer-related kinases in U373 (A) and SNB19 (B). Each array includes 2 technical replicates; 2 and 1 biological replicates were used for U373 and SNB19, respectively. Fold changes between phosphorylation levels of proteins in shWNT6 vs. shCtrl cells are shown (mean ± SD). HSP60 was used as reference protein.
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A systems administrator has recently purchased and installed a large electronic signage screen for the company’s parking garage. The screen’s management software was installed on a server with public IP address to allow remote management. The systems administrator is now troubleshooting an issue with the screen displaying unknown, random, and inappropriate messages.
A network technician receives a call from a user in the accounting department stating that Internet connectivity has been lost after receiving a new workstation. No other users in accounting are reporting similar issues. The network technician is able to ping the machine from the accounting department’s router, but is not able to ping the machine from the IT network.
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A web-based front end for a business intelligence application uses pass-through authentication to authenticate users. The application then uses a service account, to perform queries and look up data m a database A security analyst discovers employees are accessing data sets they have not been authorized to use.
The backup server connects to a NAS device using block-level storage over Ethernet. The performance is very slow, however, and the network technician suspects the performance issues are network related.
So yeah, in the following posts I'll post a chronological guide in which order to read all SOTF threads avoiding overlaps. This WIP project will take some time to finish though, as I have to convert every thread link to the new board, but I hope some of you who are interested in reading SOTF in one go/chronologically. It takes time, but I confirm that it's fun. I also hope this new project will motivate me to finally finish v3 after 5 years and read the other versions completely as well.
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Homoscedasticity was verified with Levene's test and differences between groups were assessed by a two-sided unpaired t-test, with Welch's correction applied accordingly. For the wound healing assay and radiation treatment sensitivity assay, a two-way ANOVA followed by the post-hoc Sidak's test for multiple comparison testing was used. For the TMZ treatment cell death assay, a two-way ANOVA followed by the post-hoc Tukey's test for multiple comparison testing was used. The correlation between the expressions of WNT6 and stem-related genes (from the gene ontology gene-set GO_Positive_regulation_of_stem_cell_proliferation) was calculated using Pearson's correlation coefficient (r). The effects of WNT6 on mouse OS was assessed by log-rank test.
Hazard ratios and 95% confidence intervals from the Cox univariate or, when possible, multivariate analyses were used. A random effects statistical model was applied.
A network technician needs to separate a web server listening on port 80 from the internal LAN and secure the server from the public Internet. The web server should be accessible to the public Internet over port 80 but not the private LAN.
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A technician is setting up a direct connection between two older PCs so they can communicate but not be on the corporate network. The technician does not have access to a spare switch but does have spare Cat 6 cables, RJ-45 plugs, and a crimping tool. The technician cuts off one end of the cable.
A contractor is setting up and configuring conference rooms for a convention. The contractor sets up each room in the conference center to allow wired Internet access going to individual tables. The contractor measured the distance between the hotel’s patch panel to the jack, and the distance is within Cat 5e specifications. The contractor is concerned that the room will be out of specification if cables are run in each room from the wall jacks.
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Clients are connected to a switch on the gigabit Ethernet interface; the ISP is connected to the Serial 0 interface. When the router configuration is complete and client devices are reconfigured, all clients report that they are unable to connect to the Internet.
A company’s Chief Information Security Officer (CISO) is concerned about the integrity of some highly confidential files. Any changes to these files must be tied back to a specific authorized user’s activity session.
A network technician is deploying mobile phones for a company’s sales staff. Salespeople frequently travel nationally and internationally to meet with clients, and often have to roam or switch cellular providers.
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A security analyst is investigating a malware infection that occurred on a Windows system. The system was not connected to a network and had no wireless capability Company policy prohibits using portable media or mobile storage. The security analyst is trying to determine which user caused the malware to get onto the system.
A security analyst on the threat-hunting team has developed a list of unneeded, benign services that are currently running as part of the standard OS deployment for workstations. The analyst will provide this list to the operations team to create a policy that will automatically disable the services for all workstations in the organization.
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Users are reporting Internet connectivity issues. The network administrator wants to determine if the issues are internal to the company network or if there is an issue with the ISP.
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The Chi-square test was used to assess the difference between the distributions of tumors with high and low WNT6 expression stratified for LGGs and GBMs. To evaluate the prognostic value of WNT6 expression, univariate or multivariate analyses of survival were performed using, respectively, the log-rank test or the Cox proportional hazard model, where the potential confounding effect of some variables is considered.
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A network technician is troubleshooting an end-user connectivity problem. The network technician goes to the appropriate IDF but is unable to identify the appropriate cable due to poor labeling.
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An office network consists of one two-port router connected to a 12-port switch. A four-port hub is also connected to the switch.
An organization wants to move non-essential services into a cloud computing environment. Management has a cost focus and would like to achieve a recovery time objective of 12 hours.
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A network technician wants to remotely and securely access the desktop of a Linux workstation. The desktop is running remote control software without encryption.
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Cell viability was determined 6 days after plating by both trypan blue and MTT (U373 and SNB19 cells) or MTS (U87 cells) assays. For trypan blue assay, cells were plated, in triplicate, at an initial density of 20 000 cells/well in 6-well plates. For MTT/MTS assay, transfected cells were plated in 12-well plates, in triplicate, at an initial density of 6 000 (for U373 and U87 cells) or 10 000 cells/well (SNB19).
A network engineer is configuring wireless access for guests at an organization. Access to other areas in the organization should not be accessible to guests.
A web developer wants to create a new web part within the company website that aggregates sales from individual team sites. A cybersecurity analyst wants to ensure security measurements are implemented during this process.
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A network engineer is designing a new network for a remote site. The remote site consists of ten desktop computers, ten VoIP phones, and two network printers. In addition, two of the desktop computers at the remote site will be used by managers who should be on a separate network from the other eight computers.
The annealing temperature was 60 °C for both. The levels of WNT1 and WNT3a were assessed using Taqman gene expression assays (Hs00180529_m1 and Hs00263977_m1, respectively) and normalized against TBP (Hs00427620_m1), following the manufacturer's instructions. Levels were determined based on the 2-∆∆Ct method, as previously described .
A technician logs onto a system using Telnet because SSH is unavailable. SSH is enabled on the target device, and access is allowed from all subnets. The technician discovers a critical step was missed.
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A network administrator has a monitoring system in place that is currently polling hundreds of network devices at regular intervals. The continuous polling is causing high CPU utilization on the server.
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A total of 5×105 U373 or 2×105 U87 transfected cells (resuspended in 5 µL PBS 1x) were injected into the brain striatum using a 10 µL Hamilton syringe with a point style 4 beveled 26s-gauge needle, as previously described . A total of 8 male and 6 female mice per group were used for U373 and U87 transfected cells, respectively. U373 and U87 cells were used as no reports were found for SNB19 tumorigenic capacity when orthotopically injected in immunocompromised mice. Mice were maintained under standard laboratory conditions, which included an artificial 12 h light/dark cycle, controlled ambient temperature (21 ± 1 °C) and a relative humidity of 50-60%. The confirmation of specified pathogen-free health status of sentinel mice maintained within the same animal room was performed according to FELASA guidelines. Experimental mice were always manipulated in a flow hood chamber, except during the surgery. Mice's body weight was evaluated 3 times per week, while general behavior and symptomatology were registered daily. Sacrifice at the pre-established humane endpoint was performed when mice presented severe weight loss (> 30% from maximum weight). Death was used for Kaplan-Meyer representation.
As a result, the systems administrator upgrades outdated service applications and validates the endpoint configuration against an industry benchmark. The analyst suggests developers receive additional training on implementing identity and access management, and also implements a WAF to protect against SOL injection attacks.
Users are reporting their network is extremely slow. The technician discovers pings to external host have excessive response times.
A network technician is configuring a wireless network at a branch office. Users at this office work with large files and must be able to access their files on the server quickly.
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A team of security analysts has been alerted to potential malware activity. The initial examination indicates one of the affected workstations is beaconing on TCP port 80 to five IP addresses and attempting to spread across the network over port 445.
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A technician has racked a new access switch and has run multimode fiber to a new location. After installing an extended-range 10Gb SFP in the core switch, the technician installed a 10Gb SFP in the access switch and connected the port to the new extension with a fiber jumper.
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After a server outage, a technician discovers that a physically damaged fiber cable appears to be the problem. After replacing the cable, the server will still not connect to the network. Upon inspecting the cable at the server end, the technician discovers light can be seen through one of the two fiber strands.